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src kinase activity detection kit  (Genmed Inc)

 
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    Genmed Inc src kinase activity detection kit
    A The effect of matrine on <t>Src</t> <t>kinase</t> <t>activity</t> in cancer cells, including HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa, were determined according to the instructions of Src kinase activity <t>detection</t> <t>kit.</t> Results are representative of three experiments (* P < 0.05, ** P < 0.01). B , C Schematic diagram of matrine and ATP bound to Src kinase domain. Matrine (green) and ATP (bule) molecules were depicted as the ball-and-stick models. D Dose–response curves of matrine against Src kinase at various ATP concentrations. Data are the mean ± SD of three independent experiments. E HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa cells were exposed to matrine at its optimal concentrations for 24 h, respectively. The effects of matrine on the p-Src (Tyr419) were analyzed by western blot, respectively. β-Actin was used as a control.
    Src Kinase Activity Detection Kit, supplied by Genmed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/src kinase activity detection kit/product/Genmed Inc
    Average 90 stars, based on 1 article reviews
    src kinase activity detection kit - by Bioz Stars, 2026-04
    90/100 stars

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    1) Product Images from "Src acts as the target of matrine to inhibit the proliferation of cancer cells by regulating phosphorylation signaling pathways"

    Article Title: Src acts as the target of matrine to inhibit the proliferation of cancer cells by regulating phosphorylation signaling pathways

    Journal: Cell Death & Disease

    doi: 10.1038/s41419-021-04221-6

    A The effect of matrine on Src kinase activity in cancer cells, including HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa, were determined according to the instructions of Src kinase activity detection kit. Results are representative of three experiments (* P < 0.05, ** P < 0.01). B , C Schematic diagram of matrine and ATP bound to Src kinase domain. Matrine (green) and ATP (bule) molecules were depicted as the ball-and-stick models. D Dose–response curves of matrine against Src kinase at various ATP concentrations. Data are the mean ± SD of three independent experiments. E HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa cells were exposed to matrine at its optimal concentrations for 24 h, respectively. The effects of matrine on the p-Src (Tyr419) were analyzed by western blot, respectively. β-Actin was used as a control.
    Figure Legend Snippet: A The effect of matrine on Src kinase activity in cancer cells, including HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa, were determined according to the instructions of Src kinase activity detection kit. Results are representative of three experiments (* P < 0.05, ** P < 0.01). B , C Schematic diagram of matrine and ATP bound to Src kinase domain. Matrine (green) and ATP (bule) molecules were depicted as the ball-and-stick models. D Dose–response curves of matrine against Src kinase at various ATP concentrations. Data are the mean ± SD of three independent experiments. E HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa cells were exposed to matrine at its optimal concentrations for 24 h, respectively. The effects of matrine on the p-Src (Tyr419) were analyzed by western blot, respectively. β-Actin was used as a control.

    Techniques Used: Activity Assay, Western Blot, Control

    Src promotes the proliferation of cancer cells via the MAPK/ERK, JAK2/STAT3, and PI3K/Akt phosphorylation signaling pathways. Matrine may target Src protein and inhibit its kinase activity to inhibit the proliferation of cancer cells by blocking the related phosphorylation signaling pathways.
    Figure Legend Snippet: Src promotes the proliferation of cancer cells via the MAPK/ERK, JAK2/STAT3, and PI3K/Akt phosphorylation signaling pathways. Matrine may target Src protein and inhibit its kinase activity to inhibit the proliferation of cancer cells by blocking the related phosphorylation signaling pathways.

    Techniques Used: Phospho-proteomics, Protein-Protein interactions, Activity Assay, Blocking Assay



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    Image Search Results


    A The effect of matrine on Src kinase activity in cancer cells, including HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa, were determined according to the instructions of Src kinase activity detection kit. Results are representative of three experiments (* P < 0.05, ** P < 0.01). B , C Schematic diagram of matrine and ATP bound to Src kinase domain. Matrine (green) and ATP (bule) molecules were depicted as the ball-and-stick models. D Dose–response curves of matrine against Src kinase at various ATP concentrations. Data are the mean ± SD of three independent experiments. E HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa cells were exposed to matrine at its optimal concentrations for 24 h, respectively. The effects of matrine on the p-Src (Tyr419) were analyzed by western blot, respectively. β-Actin was used as a control.

    Journal: Cell Death & Disease

    Article Title: Src acts as the target of matrine to inhibit the proliferation of cancer cells by regulating phosphorylation signaling pathways

    doi: 10.1038/s41419-021-04221-6

    Figure Lengend Snippet: A The effect of matrine on Src kinase activity in cancer cells, including HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa, were determined according to the instructions of Src kinase activity detection kit. Results are representative of three experiments (* P < 0.05, ** P < 0.01). B , C Schematic diagram of matrine and ATP bound to Src kinase domain. Matrine (green) and ATP (bule) molecules were depicted as the ball-and-stick models. D Dose–response curves of matrine against Src kinase at various ATP concentrations. Data are the mean ± SD of three independent experiments. E HT-29, MCF7, A549, BxPC-3, SKOV3, and HeLa cells were exposed to matrine at its optimal concentrations for 24 h, respectively. The effects of matrine on the p-Src (Tyr419) were analyzed by western blot, respectively. β-Actin was used as a control.

    Article Snippet: The activity of Src kinase was measured according to the instructions of Src kinase activity detection kit (GENMED Scientifics Inc., USA).

    Techniques: Activity Assay, Western Blot, Control

    Src promotes the proliferation of cancer cells via the MAPK/ERK, JAK2/STAT3, and PI3K/Akt phosphorylation signaling pathways. Matrine may target Src protein and inhibit its kinase activity to inhibit the proliferation of cancer cells by blocking the related phosphorylation signaling pathways.

    Journal: Cell Death & Disease

    Article Title: Src acts as the target of matrine to inhibit the proliferation of cancer cells by regulating phosphorylation signaling pathways

    doi: 10.1038/s41419-021-04221-6

    Figure Lengend Snippet: Src promotes the proliferation of cancer cells via the MAPK/ERK, JAK2/STAT3, and PI3K/Akt phosphorylation signaling pathways. Matrine may target Src protein and inhibit its kinase activity to inhibit the proliferation of cancer cells by blocking the related phosphorylation signaling pathways.

    Article Snippet: The activity of Src kinase was measured according to the instructions of Src kinase activity detection kit (GENMED Scientifics Inc., USA).

    Techniques: Phospho-proteomics, Protein-Protein interactions, Activity Assay, Blocking Assay